cDNA synthesis

WizScript™ RT FDmix

WizScript™ RT FDmix (dT20 or Hexamer) is a complete system for the efficient synthesis of the first-strand cDNA from RNA templates and combines all the reagents necessary for successful cDNA synthesis in a  convenient individually aliquot and lyophilized in single-tube, one-step format. The FDmix formulation saves time and reduces potential contaminating errors by eliminating several pipetting steps.  WizScript™ RT FDmix included MMLV RTase (RNase H-) which is an RNA-dependent DNA polymerase that is used in cDNA synthesis with long RNA templates. The lack of RNase H activity is important in this application in that RNase H activity will start to degrade the template during long incubation times which are required for producing long cDNAs. RNase H minus RT enables the preparation of long cDNAs and libraries containing a high percentage of full-length cDNA. An enhanced buffer allows for RT reaction temperatures up to 50°C.