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  • RT-PCR

    WizPure™ RT-PCR FDmix

    WizPure™ RT-PCR FDmix combines all the reagents necessary for successful routine RT-PCR in a convenient individually aliquot and lyophilized in single-tube, one-step format. The premixed formulation saves time and reduces potential contaminating errors by eliminating several pipetting steps.
    WizPure™ RT-PCR FDmix produces fast, highly-specific, and ultra-sensitive one-step RT-PCR, from either total RNA or mRNA using gene-specific primers. An enhanced buffer allows for RT reaction temperatures up to 50°C. This can improve the detection of more difficult targets as higher RT temperatures reduce nonspecific priming and facilitate the melting of RNA secondary structures. In addition, the RT-PCR FDmix includes the MMLV RTase (RNase H-) and antibody-based hot-start Taq DNA polymerase, which has been shown to improve the sensitivity and specificity for certain targets.

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    • Overview
      • Including loading dye for easy gel electrophoresis
      • A-tail formation at 3’ ends of amplified duplex DNA
      • Ready-to-use dried type
      • Easy reaction setup (pre-aliquot in 8-strip tube)
      • High PCR specificity and high sensitivity PCR amplification
      • Room-temperature reaction set-up
      • Contains loading dye for visual confirmation and quick electrophoresis
      • Minimal optimization requirements
      • Eliminating extra pipetting steps and reducing contamination risk from multiple reagents and providing better reproducibility
    • Ordering Information
      Cat No. Size Remark
      W2601 96 T 8 strip x 12 ea
      W2601-5 480 T 8 strip x 60 ea
    • Technical Data
    • Applications
      • High through-put RT-PCR
      • Routine RT-PCR amplification of RNA templates
      • Multiple band detection or genotyping
    • Storage & Shipping
      • Store at -20ºC, upon receipt