WizPure™ Pfu DNA Polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeum Pyrococcus furiosus. The enzyme catalyzes the template-dependent polymerization of nucleotides into duplex DNA in the 5'→3' direction.
Pfu DNA Polymerase also possesses 3'→5'exonuclease (proofreading) activity. WizPure™ Pfu DNA Polymerase exhibits the lowest error rate of any thermostable DNA polymerase studied, is even up to tenfold more accurate than normal Taq DNA polymerase. Consequently, WizPure™ Pfu DNA Polymerase is useful for polymerization reactions requiring high-fidelity synthesis.
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Cat No. | Size | Remark | Contents | |
W1330E | 250 U | without dNTPs | - 1 x 100µl, Pfu Polymerase (2.5U/µl) - 1 x 1ml, 10X Pfu Buffer |
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W1331E | 500 U | without dNTPs | - 1 x 200µl, Pfu Polymerase (2.5U/µl) - 2 x 1ml, 10X Pfu Buffer |
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W1331E-5 | 2,500 U | without dNTPs | - 1 x 1,000µl, Pfu Polymerase (2.5U/µl) - 8 x 1ml, 10X Pfu Buffer |
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W1330 | 250 U | with dNTPs | - 1 x 100µl, Pfu Polymerase (2.5U/µl) - 1 x 1ml, 10X Pfu Buffer - 1 x 0.5ml, dNTP mix (2.5 mM each) |
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W1331 | 500 U | with dNTPs | - 1 x 200µl, Pfu Polymerase (2.5U/µl) - 2 x 1ml, 10X Pfu Buffer - 1 x 1ml, dNTP mix (2.5 mM each) |
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W1331-5 | 2,500 U | with dNTPs | - 1 x 1,000µl, Pfu Polymerase (2.5U/µl) - 8 x 1ml, 10X Pfu Buffer - 5 x 1ml, dNTP mix (2.5 mM each) |