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  • Real-Time PCR > Solution mix

    WizPure™ qPCR Master-UDG (PROBE)

    WizPure™ qPCR Master-UDG (PROBE) is a ready-to-use 2X reagent ideal for most quantitative real-time PCR applications. The master mix is recommended for use with Labeled Fluorescent Probes, e.g. for 5’-Nuclease Assays or Hybridization probes. The qPCR Master-UDG (PROBE) contains an antibody-mediated hot-start Taq DNA Polymerase, MgCl2, dATP, dCTP, dGTP, dUTP, Uracil DNA Glycosylase (UDG), enhancer, and stabilizer. 
    UDG and dUTP are included in the mixture to prevent the reamplification of carryover PCR products between reactions. dUTP in the mix ensures that any amplified DNA will contain uracil. UDG removes uracil residues from single or double-stranded DNA, preventing dU-containing DNA from serving as a template in future PCRs.

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    • Overview
      • Ready-to-use solution
      • Easy reaction setup
      • Minimal optimization requirements
      • Antibody-mediated hot-start PCR
      • For universal Real-time PCR master mix
      • Suitable for most Real-time PCR machine
      • Applied unique enhancing & stabilizing technology
    • Ordering Information
      Cat No. Size Remark Contents
      W1702 1 ml 100 rxn   - 1ml, qPCR Master-UDG (PROBE)
      W1702-5 5 x 1 ml 500 rxn   - 5 x 1ml, qPCR Master-UDG (PROBE)
      W1702R 1 ml 100 rxn   - 1ml, qPCR Master-UDG (PROBE)
        - 50μl, ROX Dye (50X)
      W1702R-4 4 x 1 ml 400 rxn   - 4 x 1ml, qPCR Master-UDG (PROBE)  
        - 200μl, ROX Dye (50X)
    • Technical Data
    • Applications
      • High through-put Real-time PCR
      • Gene expression profiling
      • Gene knockdown verification
      • Array validation
      • Bacterial & Viral detection
      • Diagnostic purpose
    • Storage & Shipping
      • Store at -20ºC in a frost-free freezer
      • Shipped on ice packs and/or dry ice