WizPure™ qPCR Master (SYBR) with ROX Dye is an optimized ready-to-use solution for real-time quantitative PCR assays. It comprises all the components necessary to perform qPCR: antibody-mediated hot-start Taq DNA Polymerase, ultra-pure dNTPs, MgCl2, SYBR Green I, enhancer, and stabilizer. Hot start DNA Polymerase is activated by a 5-minute incubation step at 95°C. This prevents the extension of nonspecifically annealed primers and primer-dimers formed at low temperatures during qPCR setup. The kit includes the components necessary for performing PCR amplification and has been successfully used to amplify and detect a variety of DNA targets such as genomic DNA, cDNA, and plasmid DNA.
WizPure™ qPCR Master (SYBR) Lo-ROX kit and WizPure™ qPCR Master (SYBR) Hi-ROX kit are optimized ready-to-use 2X master mix solution for real-time quantitative PCR assays. It comprises all the components necessary to perform qPCR: antibody hot-start Taq DNA Polymerase, ultrapure dNTPs, MgCl2, SYBR Green I, enhancer, stabilizer, and ROX reference dye ensures that the kits deliver fast, highly-specific and ultra-sensitive real-time PCR.
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Cat No. | Size | Remark | Contents | |
W1711 | 1 ml | 100 rxn | - 1ml, qPCR Master (SYBR) | |
W1711-5 | 5 x 1 ml | 500 rxn | - 5 x 1ml, qPCR Master (SYBR) | |
W1711R | 1 ml | 100 rxn with ROX dye | - 1ml, qPCR Master (SYBR) - 50μl, ROX Dye (50X) |
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W1711R-4 | 4 x 1 ml | 400 rxn with ROX dye | - 4 x 1ml, qPCR Master (SYBR) - 200μl, ROX Dye (50X) |
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W1711RL | 1 ml | 100 rxn, Low ROX dye | - 1ml, qPCR Master (SYBR) Lo-ROX | |
W1711RL-5 | 5 x 1 ml | 500 rxn, Low ROX dye | - 5 x 1ml, qPCR Master (SYBR) Lo-ROX | |
W1711RH | 1 ml | 100 rxn, High ROX dye | - 1ml, qPCR Master (SYBR) Hi-ROX | |
W1711RH-5 | 5 x 1 ml | 500 rxn, High ROX dye | - 5 x 1ml, qPCR Master (SYBR) Hi-ROX |