WizPure™ qPCR Master (EVA) is an optimized ready-to-use solution for real-time quantitative PCR assays, incorporating EvaGreen dye. It comprises all the components necessary to perform qPCR: WizPure™ HS-Taq DNA Polymerase, ultrapure dNTPs, MgCl2, and EvaGreen dye. The user simply needs to add water, template, and primers. Hot start DNA Polymerase is activated by a 5 minutes incubation step at 95°C. This prevents the extension of nonspecifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
The kit includes the components necessary for performing PCR amplification and has been successfully used to amplify and detect a variety of DNA targets such as genomic DNA, cDNA, and plasmid DNA.
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| Cat No. | Size | Remark | Contents | |
| W1721 | 1 ml | 100 rxn | - 1ml, qPCR Master (EVA) | |
| W1721-5 | 5 x 1 ml | 500 rxn | - 5 x 1ml, qPCR Master (EVA) | |
| W1721R | 1 ml | 100 rxn with ROX dye | - 1ml, qPCR Master (EVA) - 50μl, ROX Dye (50X) |
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| W1721R-4 | 4 x 1 ml | 400 rxn with ROX dye | - 4 x 1ml, qPCR Master (EVA) - 200μl, ROX Dye (50X) |
