WizScript III Reverse Transcriptase

WizScript III Reverse Transcriptase

WizScript™ Reverse Transcriptase is a genetically modified M-MLV Reverse Transcriptase which exhibits RNA or DNA dependent DNA polymerase, but lacks ribonuclease H activity. This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA or DNA templates. Removal of the RNase H activity results in an increase of full-length cDNA products. The RNase H activity degrades the RNA part of the cDNA/RNA hybrid following reverse transcription, which may prevent its inhibition during subsequent PCR steps.

  • Kit Contents

    • WizScript™ Reverse Transcriptase, (200 U/μl)
    • 5X Reaction Buffer
  • Applications

    • Gene synthesis
    • First-strand synthesis of cDNA
    • RT-PCR
    • Random priming reactions
    • Library construction
    • Probe labeling
    • mRNA 5’-end mapping
  • Unit Definition

    1 unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into acid insoluble material in 10 minutes at 37°C using poly r(A)/oligo (dT) as a substrate.

  • Shipping and Storage Conditions

    • Store all components at -20ºC in a non-frost-free freezer.
  • Ordering Information

    Cat No. Size Note
    W2303 10,000 U  
    W2303-5 50,000 U  
  • Manual

Size

ADDRESS

A-1806 Woolim Lions Valley 2, Sagimakgol-ro 45beon-gil 14, Jungwon-gu, Seongnam,

South Korea 13209

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sales@wizbiosolution.com

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