cDNA Synthesis Kit

cDNA Synthesis Kit

WizScript™ cDNA Synthesis Kit is a complete system for the efficient synthesis of first strand cDNA from RNA templates. The recombinant Ribonuclease Inhibitor, supplied with the kit effectively protects RNA template from degradation. The kit is also supplied with both oligo dT and random primers. The oligo dT anneals selectively on the poly(A) tail of mRNA. Random primers do not require the presence of poly(A). Therefore, they can be used for transcription of the 5’-end regions of mRNA. Gene-specific primers may also be used with the kit. The first strand of cDNA can be directly used as a template in PCR.
  • Kit Contents

    • WizScript™ RTase (200U/µl)
    • 10X Reaction Buffer
    • RNase Inhibitor (40U/µl)
    • dNTP mix (2.5mM)
    • Oligo dT20 primer (50pM)
    • Random hexamer (50pM)
    • DTT (100 mM)
    • RNase free water
  • Applications

    • First Strand cDNA synthesis for RT-PCR
    • Construction of cDNA libraries
    • Generation of probes for hybridization
    • Antisense RNA synthesis
  • Shipping and Storage Conditions

    • Store all components at -20ºC in a non-frost-free freezer.
  • Ordering Information

    Cat No. Size Note
    W2201 50 Reactions  
    W2202 100 Reactions  
  • Manual

Size

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