CrystalMix™ RT-PCR kit contained all the reagents necessary for successful one-step RT-PCR in a convenient individually aliquot and freeze-dried format in an 8-strip PCR tube. The CrystalMix™ RT-PCR kit is an optimized, ready-to-use RT-PCR premixture including enhancers, stabilizers, and ambient temperature stable format. There is no need for freezing, thawing steps, or pipetting on ice, so minimized the risk of human errors and contaminations. CrystalMix™ RT-PCR kit produces fast, highly-specific, and sensitive one-step RT-PCR, from either total RNA or mRNA using gene-specific primers. An enhanced buffer allows for RT reaction temperatures up to 50°C. This can improve the detection of more difficult targets as higher RT temperatures reduce nonspecific priming and facilitate the melting of RNA secondary structures.
In addition, the CrystalMix™ RT-PCR kit includes the hot-start Taq DNA polymerase, which has been shown to improve the sensitivity and specificity for certain targets.
RT-PCR converts and amplifies a single-stranded RNA template yielding a double-stranded DNA product. In the RT step, reverse transcriptase synthesizes single-stranded DNA molecules complementary to the RNA template (first-strand cDNA). During the PCR step, a thermostable DNA polymerase first synthesizes second-strand DNA complementary to the first-strand cDNA molecules. This generates a double-stranded DNA template which is exponentially amplified in subsequent rounds of thermal cycling. RT-PCR reactions can be directly loaded onto an agarose gel without the additional need of loading buffer and dyes.
- CrystalMix™ RT-PCR mix in 8-strip PCR tube
- Routine and one-step RT-PCR amplification of RNA templates
- Multiple band detection or genotyping
- Molecular diagnostics
Shipping and Storage Conditions
- Store at below 25°C in the airtight pouch with the desiccant
Cat No. Size Note W8201 96 rxn 0.2ml, Clear 8-strip tube