FX-Taq DNA Polymerase

FX-Taq DNA Polymerase

WizPure™ FX-Taq DNA Polymerase is a thermally stable, processive, 5’→ 3’ DNA polymerase and 3’→5’ proofreading function. The FX-Taq DNA polymerase optimized for PCR amplification of genomic DNA templates up to 20 kb and lambda DNA up to 30 kb. With its enhanced processivity, yield, speed and excellent 3’→5’ exonuclease and 3′→5′proofreading activity, this enzyme is able to consistently deliver accurate and reliable amplification of long templates. This product is the ideal choice for long DNA templates unable to be amplified in conventional PCR, and is highly suitable for multiple downstream applications including complex cloning and genotyping experiments. The PCR product amplified with this mixture has one A added at 3’-end, so the product can be directly used for TA cloning.
  • Kit Contents

    • WizPure™ FX-Taq DNA Polymerase (2.5U/μl)
    • 10X Reaction Buffer
    • dNTP mix (2.5mM each)
    • 25mM MgCl2
  • Applications

    • Long range PCR
    • High-fidelity PCR
    • Primer-extension reaction
    • Genotyping
    • Library construction
    • High GC amplification
    • Next-generation DNA sequencing
    • TA cloning
  • Unit Definition

    1 unit is defined as the amount of enzyme that will incorporate 10 nmol of
    dNTP into acid-insoluble material in 30 minutes at 75°C.

  • Shipping and Storage Conditions

    • Store all components at -20ºC in a non-frost-free freezer.
  • Ordering Information

    Cat No. Size Note
    W1341ES 250 U without dNTPs
    W1341E 500 U without dNTPs
    W1341E-5 2,500 U without dNTPs
    W1341S 250 U with dNTPs
    W1341 500 U with dNTPs
    W1341-5 2,500 U with dNTPs
  • Manual



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