Gel/PCR Purification Kit

Gel/PCR Purification Kit

The WizPrep™ Gel/PCR Purification Mini Kit was designed to recover or concentrate DNA fragments (100 bp→10 Kb) from agarose gel, PCR, or other enzymatic reactions. Chaotropic salt is used to dissolve agarose gel and denature enzymes. DNA fragments in the chaotropic salt are bound by the glass fiber matrix of the spin column.
The unique dual purpose application and high yield DNA/minicolumn make this kit an exceptional value. The method uses a chaotropic salt, guanidine thiocyanate to dissolve agarose gel and denature enzymes. DNA fragments in chaotropic salt solution bind to the glass fiber matrix of the spin column. Following washing off of contaminants, the purified DNA fragments are eluted by addition of low salt elution buffer or water. Salts, enzymes and unincorporated nucleotides are effectively removed from reaction mixtures without phenol extraction or alcohol precipitation.

  • Kit Contents

    Contents

    100 prep

    300 prep

    GP Buffer

    60 ml

    90 ml

    Wash Buffer

    16 ml

    25 ml

    Elution Buffer

    10 ml

    30 ml

    DNA Spin Column

    100 pcs

    300 pcs

    Collection Tube

    100 pcs

    300 pcs

  • Applications

    • Gel elution
    • PCR clean up

     

  • Shipping and Storage Conditions

    • Room temperature
  • Ordering Information

    Cat No. Size Note
    W70150-100 100 prep  
    W70150-300 300 prep  
  • Manual

Size

ADDRESS

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