Gel/PCR Purification Kit
The WizPrep™ Gel/PCR Purification Mini Kit was designed to recover or concentrate DNA fragments (100 bp→10 Kb) from agarose gel, PCR, or other enzymatic reactions. Chaotropic salt is used to dissolve agarose gel and denature enzymes. DNA fragments in the chaotropic salt are bound by the glass fiber matrix of the spin column.
The unique dual purpose application and high yield DNA/minicolumn make this kit an exceptional value. The method uses a chaotropic salt, guanidine thiocyanate to dissolve agarose gel and denature enzymes. DNA fragments in chaotropic salt solution bind to the glass fiber matrix of the spin column. Following washing off of contaminants, the purified DNA fragments are eluted by addition of low salt elution buffer or water. Salts, enzymes and unincorporated nucleotides are effectively removed from reaction mixtures without phenol extraction or alcohol precipitation.
DNA Spin Column
- Gel elution
- PCR clean up
Shipping and Storage Conditions
- Room temperature
Cat No. Size Note W70150-100 100 prep W70150-300 300 prep