HS-PCR FDmix (UDG)
WizPure™ HS-PCR FDmix (UDG) combines all the reagents necessary for successful routine hot-start PCR in a convenient individually aliquot and lyophilized in single-tube. WizPure™ HS-PCR FDmix (UDG) is an economical, highly efficient and ready-to-use PCR premix of WizPure™ HS-Taq DNA Polymerase, PCR buffer, MgCl2 and dATP, dCTP, dGTP, dUTP and Uracil DNA Glycosylase (UDG), except DNA template and primers. UDG and dUTP are included in the mixture to prevent the reamplification of carryover PCR products between reactions. dUTP in the mix ensures that any amplified DNA will contain uracil. UDG removes uracil residues from single- or double-stranded DNA, preventing dU-containing DNA from serving as template in future PCRs. Incubation of subsequent PCRs with UDG before cycling destroys any contaminating dU-containing PCR product from previous reactions.
Kit Contents
- HS-PCR FDmix (UDG) in 8 strip PCR tube
Applications
- High through-put PCR
- Routine diagnostic PCR requiring high reproducibility
- Hot-start PCR
- Primer extension
- Multiplex PCR
- High specific amplification of DNA fragments shorter than 3kb.
Shipping and Storage Conditions
- Store all components at -20ºC in a non-frost-free freezer.
- Shipping at room temperature has no detrimental effects on the quality of this reagent.
Ordering Information
Cat No. Size Note W1612 96 T Prevent Carryover Contamination W1612-5 480 T Prevent Carryover Contamination Manual