MMLV RTase (RNase H+)

MMLV RTase (RNase H+)

WizPure™ MMLV RTase (RNase H+) encoded by Moloney Murine Leukemia Virus (MMLV RT) is an RNA-dependent DNA polymerase that synthesizes the complementary cDNA first strand from a single-stranded RNA template to which  a primer has been hybridized. This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA or DNA templates. MMLV RTase will also extend primers hybridized to single-stranded DNA. Second strand cDNA synthesis can be achieved from some mRNA templates without an additional DNA polymerase.
  • Kit Contents

    • WizPure™ MMLV RTase (RNase H+), (200 U/μl)
    • 5X Reaction Buffer
  • Applications

    • Gene synthesis
    • First-strand synthesis of cDNA
    • RT-PCR
    • Random priming reactions
    • Library construction
    • Probe labeling
    • mRNA 5’-end mapping
  • Unit Definition

    1 unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into acid insoluble material in 10 minutes at 37°C using poly r(A)/oligo (dT) as a substrate.

  • Shipping and Storage Conditions

    • Store all components at -20ºC in a non-frost-free freezer.
  • Ordering Information

    Cat No. Size Note
    W2301 10,000 U  
    W2301-5 50,000 U  
  • Manual



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