MMLV RTase (RNase H+)
WizPure™ MMLV RTase (RNase H+) encoded by Moloney Murine Leukemia Virus (MMLV RT) is an RNA-dependent DNA polymerase that synthesizes the complementary cDNA first strand from a single-stranded RNA template to which a primer has been hybridized. This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA or DNA templates. MMLV RTase will also extend primers hybridized to single-stranded DNA. Second strand cDNA synthesis can be achieved from some mRNA templates without an additional DNA polymerase.
- WizPure™ MMLV RTase (RNase H+), (200 U/μl)
- 5X Reaction Buffer
- Gene synthesis
- First-strand synthesis of cDNA
- Random priming reactions
- Library construction
- Probe labeling
- mRNA 5’-end mapping
1 unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into acid insoluble material in 10 minutes at 37°C using poly r(A)/oligo (dT) as a substrate.
Shipping and Storage Conditions
- Store all components at -20ºC in a non-frost-free freezer.
Cat No. Size Note W2301 10,000 U W2301-5 50,000 U