Pfu 2X Master
WizPure™ Pfu 2X Master is ready-to-use Pfu DNA polymerase pre-mixes are the innovation for convenience of your routine PCR as you just have to add your template DNA besides your primers & put the tubes for amplifications. WizPure™ Pfu 2X Master is an optimized, ready-to-use PCR mixture of Pfu DNA Polymerase, PCR buffer, MgCl2 and dNTP’s. This Pfu 2X Master contains all components for PCR, except DNA template and primers. The mixture is suitable for amplification of most of the DNA templates.
In addition to 5´ to 3´ DNA polymerase activity, Pfu DNA Polymerase also possesses 3´ to 5´ exonuclease (proofreading) activity. Pfu DNA Polymerase exhibits the lowest error rate of any thermostable DNA polymerase studied, is even up to ten fold more accurate than normal Taq DNA polymerase. Consequently, Pfu DNA Polymerase is useful for polymerization reactions requiring high-fidelity synthesis.
Kit Contents
- WizPure™ Pfu 2X Master
Applications
- High fidelity PCR for cloning
- Amplification of long DNA fragments ( >5 ~ 15 kb )
- DNA amplification to generate blunt end products
- Site-directed mutagenesis
- DNA sequencing
Shipping and Storage Conditions
- Store all components at -20ºC in a non-frost-free freezer.
Ordering Information
Cat No. Size Note W1451 1 ml 100 Reactions W1451-8 8 X 1 ml 800 Reactions Manual
Size