Pfu DNA Polymerase

Pfu DNA Polymerase

WizPure™ Pfu DNA Polymerase is a highly thermostable DNA polymerasefrom the hyperthermophilic archaeum Pyrococcus furiosus. The enzymecatalyzes the template-dependent polymerization of nucleotides into duplexDNA in the 5'→3' direction. Pfu DNA Polymerase also possesses 3'→5'exonuclease (proofreading) activity. WizPure™ Pfu DNA Polymerase exhibitsthe lowest error rate of any thermostable DNA polymerase studied, is evenup to ten fold more accurate than normal Taq DNA polymerase. Consequently, WizPure™ Pfu DNA Polymerase is useful for polymerizationreactions requiring high-fidelity synthesis.

  • Kit Contents

    • WizPure™ Pfu DNA Polymerase (2.5U/μl)
    • 10X Pfu Reaction Buffer
    • dNTPs mix (2.5mM each)
  • Applications

    • High fidelity PCR for cloning
    • Amplification of long DNA fragments (>5-15 kb)
    • DNA amplification to generate blunt end products
    • Site-directed mutagenesis
    • DNA sequencing
  • Unit Definition

    1 unit of the enzyme catalyzes the incorporation of 10 nanomoles of deoxyribonucleotides into a polynucleotide fraction in 30 min at 70°C.

  • Shipping and Storage Conditions

    • Store all components at -20ºC in a non-frost-free freezer.
  • Ordering Information

    • Cat No. Size Note
      W1330E 250 U without dNTPs
      W1331E 500 U without dNTPs
      W1331E-5 2,500 U without dNTPs
      W1330 250 U with dNTPs
      W1331 500 U with dNTPs
      W1331-5 2,500 U with dNTPs
  • Manual



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