Proteinase K

Proteinase K

Proteinase K is a subtilisin-like endolytic protease that is isolated from the saprophytic fungus Tritirachium album. It has a high activity that is stable across a wide range of pH and temperature conditions and is suited to short digestion times. Proteinase K is active in a wide range of temperatures and buffers with optimal activity between 20 and 60°C and a pH between 7.5 and 12.0. Calcium is not essential to the function of proteinase K.

  • Properties

    • Form : White to Light Yellow Powder, Lyophilized from purified liquid enzyme
    • Source : Mutated gene from Tritirachium album limber, Recombinant
    • Specific Activity : ≥30U/mg dry weights
    • Molecular Weight : 29.3 kDa


  • Applications

    • Isolation of plasmid and genomic DNA
    • Isolation of RNA
    • Inactivation of RNases, DNases and enzymes in reactions
    • Removal of enzymes from DNA to improve cloning efficiency
    • PCR purification
  • Shipping and Storage Conditions

    • Store at 4 to -20ºC recommended : Proteinase K powder is stable at room temperature in two years.
    • Expiration : Three years if kept below 4ºC. 
    • Dilution Buffer: none specific, distilled water recommended.
  • Ordering Information

    Cat No. Size Note
    W6050 22 mg  
  • Manual



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