qPCR (EVA Green)

WizPure™ qPCR Master (EVA) is an optimized ready-to-use solution for real-time quantitative PCR assays, incorporating EvaGreen dye. It comprises all the components necessary to perform qPCR:  antibody based hot-start Taq DNA Polymerase, ultrapure dNTPs, MgCl2 and real-time PCR grade EvaGreen dye. The user simply needs to add water, template and primers. Hot-start DNA polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of nonspecifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.  The kits includes the components necessary for performing PCR amplification, and have been successfully used to amplify and detect a variety of DNA targets such as genomic DNA, cDNA and plasmid DNA.