qPCR (EVA Green)

qPCR (EVA Green)

WizPure™ qPCR Master (EVA) is an optimized ready-to-use solution for real-time quantitative PCR assays, incorporating EvaGreen dye. It comprises all the components necessary to perform qPCR:  antibody based hot-start Taq DNA Polymerase, ultrapure dNTPs, MgCl2 and real-time PCR grade EvaGreen dye. The user simply needs to add water, template and primers. Hot-start DNA polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of nonspecifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.  The kits includes the components necessary for performing PCR amplification, and have been successfully used to amplify and detect a variety of DNA targets such as genomic DNA, cDNA and plasmid DNA.
  • Kit Contents

    • WizPure™ qPCR Master (EVA) mix
    • ROX dye (50 X) : 1721R only
  • Applications

    • Real-time PCR
    • Gene expression profiling
    • Gene knockdown verification
    • Array validation
    • High Resolution Melt (HRM) analysis
  • Shipping and Storage Conditions

    • Store all components at -20ºC in a non-frost-free freezer.
  • Ordering Information

    Cat No. Size Note
    W1721 1 ml 100 Reactions
    W1721-8 8 X 1 ml 800 Reactions
    W1721R 1 ml 100 Reactions, with ROX dye
    W1721R-5 5 X 1 ml 500 Reactions, with ROX dye
  • Manual



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