qPCR (EVA Green)
WizPure™ qPCR Master (EVA) is an optimized ready-to-use solution for real-time quantitative PCR assays, incorporating EvaGreen dye. It comprises all the components necessary to perform qPCR: antibody based hot-start Taq DNA Polymerase, ultrapure dNTPs, MgCl2 and real-time PCR grade EvaGreen dye. The user simply needs to add water, template and primers. Hot-start DNA polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of nonspecifically annealed primers and primer-dimers formed at low temperatures during qPCR setup. The kits includes the components necessary for performing PCR amplification, and have been successfully used to amplify and detect a variety of DNA targets such as genomic DNA, cDNA and plasmid DNA.
- WizPure™ qPCR Master (EVA) mix
- ROX dye (50 X) : 1721R only
- Real-time PCR
- Gene expression profiling
- Gene knockdown verification
- Array validation
- High Resolution Melt (HRM) analysis
Shipping and Storage Conditions
- Store all components at -20ºC in a non-frost-free freezer.
Cat No. Size Note W1721 1 ml 100 Reactions W1721-8 8 X 1 ml 800 Reactions W1721R 1 ml 100 Reactions, with ROX dye W1721R-5 5 X 1 ml 500 Reactions, with ROX dye