qPCR-UDG (Probe)

WizPure™ qPCR Master-UDG (PROBE) is ready-to-use 2X reagent ideal for most quantitative Real-time PCR applications. The master mix is recommended for use with Labeled Fluorescent Probes, e.g. for 5’-Nuclease Assays or Hybrid-ization probes. The qPCR Master-UDG (PROBE) is an optimized, ready-to-use PCR mixture of antibody based hot-start Taq DNA Polymerase, PCR buffer, MgCl2 and dATP, dCTP, dGTP, dUTP and Uracil DNA Glycosylase (UDG), except DNA template and primers. UDG and dUTP are included in the mixture to prevent the reamplification of carryover PCR products between reactions. dUTP in the mix ensures that any amplified DNA will contain uracil. UDG removes uracil residues from single- or double-stranded DNA, preventing dU-containing DNA from serving as template in future PCRs. Incubation of subsequent PCRs with UDG before cycling destroys any contaminating dU-containing PCR product from previous reactions.