qRT-PCR (EVA green)

qRT-PCR (EVA green)

WizPure™ qRT-PCR Master (EVA) is an optimised ready-to-use solution for one-step quantitative RT-PCR assays, incorporating EvaGreen dye. It comprises all the components necessary to perform qRT-PCR : WizScript™ RTase, hot-start Taq DNA Polymerase, dNTPs, MgCl2 and EvaGreen dye. The user simply needs to add water, template and primers. An enhanced buffer allows for RT reaction temperatures up to 50°C. This can improve detection of more difficult targets as higher RT temperatures reduce nonspecific priming and facilitate melting of RNA secondary structures.
The kit includes the components necessary for performing RT-PCR amplification, and have been successfully used to DNA amplify and detect a variety of RNA targets. The kit provides a one-step, simple, robust, inexpensive assay for detection and quantitative analysis of gene expression directly from cells or RNA with intercalator format.

  • Kit Contents

    • WizPure™ qRT-PCR (EVA) Master mix
  • Applications

    • One-step Real-Time RT-PCR
    • Gene expression profiling
    • Gene knockdown verification
    • Array Validation
  • Shipping and Storage Conditions

    • Store all components at -20ºC in a non-frost-free freezer.
  • Ordering Information

    Cat No. Size Note
    W2721 1 ml 100 Reactions
    W2721-8 8 X 1 ml 800 Reactions
  • Manual

Size

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