qRT-PCR (Probe)

WizPure™ qRT-PCR Master (PROBE) is an optimised ready-to-use solution for one-step quantitative RT-PCR assays. The master mix is recommended for use with Labeled Fluorescent Probes, e.g. for 5’-Nuclease Assays or Hybridization probes. It comprises all the components necessary to perform qRT-PCR: MMLV RTase (RNase H-), antibody based hot-start Taq DNA Polymerase, ultrapure dNTPs and MgCl2. The user simply needs to add water, template and primers. An enhanced buffer allows for RT reaction temperatures up to 50°C. This can improve detection of more difficult targets as higher RT temperatures reduce nonspecific priming and facilitate melting of RNA secondary structures.