qRT-PCR (Probe)

qRT-PCR (Probe)

WizPure™ qRT-PCR Master (PROBE) is an optimised ready-to-use solution for one-step quantitative RT-PCR assays. The master mix is recommended for use with Labeled Fluorescent Probes, e.g. for 5’-Nuclease Assays or Hybridization probes. It comprises all the components necessary to perform qRT-PCR: MMLV RTase (RNase H-), antibody based hot-start Taq DNA Polymerase, ultrapure dNTPs and MgCl2. The user simply needs to add water, template and primers. An enhanced buffer allows for RT reaction temperatures up to 50°C. This can improve detection of more difficult targets as higher RT temperatures reduce nonspecific priming and facilitate melting of RNA secondary structures.
  • Kit Contents

    • WizPure™ qRT-PCR Master (PROBE) mix
  • Applications

    • Real-time RT-PCR
    • Gene expression profiling
    • Gene knockdown verification
    • Array validation
  • Shipping and Storage Conditions

    • Store all components at -20ºC in a non-frost-free freezer.
  • Ordering Information

    Cat No. Size Note
    W2701 1 ml 100 Reactions
    W2701-8 8 X 1 ml 800 Reactions
  • Manual



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