qRT-PCR (Super Green)

qRT-PCR (Super Green)

WizPure™ qRT-PCR Master (Super Green) is an optimised ready-to-use solution for one-step quantitative RT-PCR assays, incorporating Super GreenGreen dye. It comprises all the components necessary to perform qRT-PCR : WizScript™ RTase, hot-start Taq DNA Polymerase, dNTPs, MgCl2
and Super Green dye. The user simply needs to add water, template and primers. An enhanced buffer allows for RT reaction temperatures up to 50°C.
This can improve detection of more difficult targets as higher RT temperatures reduce nonspecific priming and facilitate melting of RNA secondary structures.

  • Kit Contents

    • WizPure™ qRT-PCR (Super Green) Master mix
  • Applications

    • One-step Real-Time RT-PCR
    • Gene expression profiling
    • Gene knockdown verification
    • Array Validation
  • Shipping and Storage Conditions

    • Store all components at -20ºC in a non-frost-free freezer.
  • Ordering Information

    Cat No. Size Note
    W2731 1 ml 100 Reactions
    W2731-8 8 X 1 ml 800 Reactions
  • Manual

Size

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