WizScript™ RT FDmix (dT20 or Hexamer) is a complete system for the efficient synthesis of first strand cDNA from RNA templates and combines all the reagents necessary for successful cDNA synthesis in a convenient individually aliquot and lyophilized in single-tube, one-step format. The FDmix formulation saves time and reduces potential contaminating errors by eliminating several pipetting steps. WizScript™ RT FDmix included MMLV RTase (RNase H-) which is an RNA-dependent DNA polymerase that is used in cDNA synthesis with long RNA templates. The lack of RNase H activity is important in this application in that RNase H activity will start to degrade template during long incubation times which are required for producing long cDNAs. RNase H minus RT enables preparation of long cDNAs and libraries containing a high percentage of full-length cDNA. And enhanced buffer allows for RT reaction temperatures up to 50°C.
- RT FDmix in 8 strip PCR tube
- First Strand cDNA synthesis for RT-PCR
- Construction of cDNA libraries
- Generation of probes for hybridization
- Antisense RNA synthesis
Shipping and Storage Conditions
- Store all components at -20ºC in a non-frost-free freezer.
Cat No. Product Size Note W2204 RT FDmix (dT20) 96 Reactions included oligo dT20 primer W2205 RT FDmix (Hexamer) 96 Reactions included Random hexamer