RT Master

RT Master

WizScript™ RT Master is a complete system for the efficient synthesis of first strand cDNA from RNA templates. WizScript™ RT Master inclueded MMLV RTase (RNase H-) which is an RNA-dependent DNA polymerase that is used in cDNA synthesis with long RNA templates. The lack of RNase H activity is important in this application in that RNase H activity will start to degrade template during long incubation times which are required for producing long cDNAs.
RNase H minus RT enables preparation of long cDNAs and libraries containing a high percentage of full-length cDNA. The kit is also supplied with both oligo dT and random primers. The oligo dT anneals selectively on the poly(A) tail of mRNA. Random primers do not require the presence of poly(A). Therefore, they can be used for transcription of the 5’-end regions of mRNA. Gene-specific primers may also be used with the kit. The first strand of cDNA can be directly used as a template in PCR.

  • Kit Contents

    • RT Master mix
    • Oligo dT20 primer (50 pM)
    • Random hexamer (50 pM)
    • RNase free water
  • Applications

    • First Strand cDNA synthesis for RT-PCR
    • Construction of cDNA libraries
    • Generation of probes for hybridization
    • Antisense RNA synthesis
  • Shipping and Storage Conditions

    • Store all components at -20ºC in a non-frost-free freezer.
  • Ordering Information

    Cat No. Size Note
    W2203 100 Reactions  
  • Manual

Size

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