RT-PCR FDmix

WizPure™ RT-PCR FDmix combines all the reagents necessary for successful routine RT-PCR in a convenient individually aliquot and lyophilized in single-tube, one-step format. The premixed formulation saves time and reduces potential contaminating errors by eliminating several pipetting steps. WizPure™ RT-PCR FDmix produces fast, highly-specific and ultra-sensitive one-step RT-PCR, from either total RNA or mRNA using gene-specific primers. An enhanced buffer allows for RT reaction temperatures up to 50°C. This can improve detection of more difficult targets as higher RT temperatures reduce nonspecific priming and facilitate melting of RNA secondary structures. In addition, the RT-PCR FDmix includes the MMLV RTase (RNase H-) and antibody based hot-start Taq DNA polymerase, which has been shown to improve the sensitivity and specificity for certain targets.