UDG (Uracil DNA Glycosylase)

UDG (Uracil DNA Glycosylase)

Uracil-DNA Glycosylase (UDG) catalyzes the hydrolysis of the N-glycosylic bond between the uracil and sugar, leaving an abasic site in uracil-containing single or double-stranded DNA. The enzyme shows no measurable activity on short oligonucleotides (<6 bases), or RNA substrates.

  • Contents

    • Control of carry-over contamination in PCR
    • Glycosylase mediated single nucleotide polymorphism detection
    • Site-directed mutagenesis
    • As a probe for protein-DNA interaction studies
    • SNP genotyping
    • Cloning of PCR products
    • Generation of single strand overhangs of PCR products and cDNA
  • Applications

    • Isolation of plasmid and genomic DNA
    • Isolation of RNA
    • Inactivation of RNases, DNases and enzymes in reactions
    • Removal of enzymes from DNA to improve cloning efficiency
    • PCR purification
  • Shipping and Storage Conditions

    • Store all components at -20ºC in a non-frost-free freezer.
  • Ordering Information

    Cat No. Size Note
    W6030 1,000 U  
    W6030-5 5,000 U  
    W6030-10 10,000 U  

     

  • Manual

Size

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