UDG (Uracil DNA Glycosylase)
Uracil-DNA Glycosylase (UDG) catalyzes the hydrolysis of the N-glycosylic bond between the uracil and sugar, leaving an abasic site in uracil-containing single or double-stranded DNA. The enzyme shows no measurable activity on short oligonucleotides (<6 bases), or RNA substrates.
Contents
- Control of carry-over contamination in PCR
- Glycosylase mediated single nucleotide polymorphism detection
- Site-directed mutagenesis
- As a probe for protein-DNA interaction studies
- SNP genotyping
- Cloning of PCR products
- Generation of single strand overhangs of PCR products and cDNA
Applications
- Isolation of plasmid and genomic DNA
- Isolation of RNA
- Inactivation of RNases, DNases and enzymes in reactions
- Removal of enzymes from DNA to improve cloning efficiency
- PCR purification
Shipping and Storage Conditions
- Store all components at -20ºC in a non-frost-free freezer.
Ordering Information
Cat No. Size Note W6030 1,000 U W6030-5 5,000 U W6030-10 10,000 U Manual
Size