WizScript™ cDNA Synthesis Kit is a complete system for the efficient synthesis of first-strand cDNA from RNA templates. The recombinant Ribonuclease Inhibitor, supplied with the kit effectively protects the RNA template from degradation. The kit is also supplied with both oligo dT and random primers. The oligo dT anneals selectively on the poly(A) tail of mRNA. Random primers do not require the presence of poly(A). Therefore, they can be used for transcription of the 5’-end regions of mRNA. Gene-specific primers may also be used with the kit. The first strand of cDNA can be directly used as a template in PCR.
Cat No. | Size | 가격(원) |
W2201 | 50 rxn | 160,000 |
W2202 | 100 rxn | 280,000 |
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Contents | 50 rxn | 100 rxn | |
WizScript™ RTase (200U/µl) | 50 µl | 100 µl | |
10X Reaction Buffer | 100 µl | 200 µl | |
RNase Inhibitor (40U/µl) | 25 µl | 50 µl | |
dNTP mix (2.5mM) | 100 µl | 200 µl | |
Oligo dT20 primer (50pM) | 50 µl | 100 µl | |
Random hexamer (50pM) | 100 µl | 200 µl | |
DTT (100 mM) | 50 µl | 100 µl | |
RNase free water | 1 ml | 1 ml |