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Key Features

• Detects Treponema pallidum in a single real-time PCR reaction
• Lyophilized ready-to-use mix — no reagent setup required
• High sensitivity and specificity
• Internal control is included to ensure assay quality
• Hot-start PCR technology minimizes non-specific PCR reactions and increases specificity.
• Uracil-DNA glycosylase (UDG) technology prevents carryover contamination.
• Compatible with major real-time PCR instruments
• Using CLEO Q16, the PCR setup can be easily set up with QR code recognition, and test results can be automatically analyzed, allowing for quick and intuitive confirmation of results.

Specifications

Principle of Procedure	Real-time PCR
Target	FAM for Treponema pallidum, and Cy5 for Internal Control
Reagent Type	Lyophilized (Freeze-dried) in a PCR tube
Limit of Detection (LoD)	1.25 copies/μL
Precision	Respectively detect the reference specimen of high and low concentrations in different time ranges-10 times, and the precision values of intra-assay and inter-assay Ct values were all <1.5%.
Specificity	Analytical specificity was evaluated for cross-reactivity by testing similar intestinal bacteria and microorganisms. The test was repeated three times and showed no cross-reactivity.
Sample Types	Vaginal swabs or urine specimens
Shipping/Storage	Shipping and Storage at room temperature for 12 months
Compatible Instruments	CLEO™ Q16 (Wizbiosolutions), CFX96 (Bio-Rad), QuantStudio™ 5 (ThermoFisher)

Protocol

Technical Data

Storage & Shipping

Room temperature (4 - 25℃)