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Key Features

• Detects Vibrio parahaemolyticus in a single real-time PCR reaction
• Lyophilized ready-to-use mix — no reagent preparation required
• High sensitivity and specificity
• Internal control included for result validation
• Hot-start PCR technology minimizes non-specific PCR reactions and increases specificity.
• Uracil-DNA glycosylase (UDG) technology prevents carryover contamination.
• Using the CLEO™ Q16 system, PCR setup is easily configured through QR code scanning, and results are automatically analyzed for fast and intuitive interpretation.

Specifications

Principle of Procedure	Real-time PCR
Target	FAM for Vibrio parahaemolyticus, and Cy5 for Internal Control
Reagent Type	Lyophilized (Freeze-dried) in a PCR tube
Limit of Detection (LoD)	2.5 copies/μL
Precision	Respectively detect the reference specimen of high and low concentrations in different time ranges-10 times, and the precision values of intra-assay and inter-assay Ct values were all <1.5%.
Specificity	Analytical specificity was evaluated for cross-reactivity by testing similar intestinal bacteria and microorganisms. The test was repeated three times and showed no cross-reactivity.
Sample Types	Enrichment samples, isolate cultures, and microbial cultures
Shipping/Storage	Shipping and Storage at room temperature for 12 months
Compatible Instruments	CLEO™ Q16 (Wizbiosolutions), CFX96 (Bio-Rad), QuantStudio™ 5 (ThermoFisher)

Protocol

Technical Data

Storage & Shipping

Room temperature (4 - 25℃)

Ordering Information

Product Name	Cat No.	Package
WizDx™ CrystalMix Vibrio para	DX1245	96 Tests